We also examined NRF1 expression profiles in diet-induced MASH model in mice and found that hepatic NRF1 expression was markedly downregulated, whereas ubiquitin expression, reflecting the degradative ability of the proteasome, was increased in liver tissues from mice fed an HFD (60% caloric intake provided by saturated fat) compared with those from mice fed an SCD for 20 weeks (Fig. 1D). This evidence concerns the gene NRF1 and metabolic dysfunction-associated steatohepatitis.