MIF and metabolic dysfunction-associated steatotic liver disease: To investigate whether fatty liver-derived MIF promote liver metastasis in vivo, we generated liver-specific knockdown of Mif using the AAV8 system, which enables precise manipulation of gene expression primarily in hepatocytes within the liver.36 To specifically investigate the role of MIF in metastasis without confounding its potential effects on MASLD development, we injected AAV8_shMif at the time of fatty liver establishment (4 weeks after CDAHFD feeding).