To study the effects of FBXO11 on RUNX1-driven MDS, we isolated c-kit+ HSPCs from Fbxo11+/+Mx1-Cre+ and Fbxo11+/flMx1-Cre+ donor mice (CD45.2+) and expressed the human ortholog mutant RUNX1 (41-214) by retroviral transduction (Figure 6H). Here, MX1 is linked to myelodysplastic syndrome.