Using a cutoff of ≥ 10 RLU, the treponemal IgM positive results obtained with the Chembio DPP Syphilis TnT Assay identified 8/9 (88.9%) neonates in the high-risk groups and 8/13 (61.5%) newborns in the CS Less Likely scenario. The gene discussed is CD40LG; the disease is syphilis.