To investigate immunological changes during the acute and recovery phases of CDI, immune cells were isolated from the colonic lamina propria and analyzed by spectral flow cytometry to identify myeloid cells (CD11b+), dendritic cells (DCs; MHC-II+CD11c+), and innate lymphoid cell subsets (ILCs; CD127+CD90+). Here, THY1 is linked to clostridium difficile infection.