HFD-fed male mice had a significant increase in CD103+ CD11b− DCs in the colonic lamia propria and showed increased transcript levels of Itgae (CD103), Xcr1, and Irf8, along with Cd40 and Cd86 in cecal tissue, suggesting the activation of cDC1 during the acute phase of the CDI. Here, CD40 is linked to clostridium difficile infection.