In melanoma, tumor antigen reactive CD4+ cells were restricted to the CXCL13+PD1high fraction57 and could be divided into several states that resemble the populations we observed here (ie. Cytotoxic CD103+LAG3+IFNg+ and Tfh memory like TCF7+, Bcl6+, CXCR5+ cells)57. This evidence concerns the gene CD4 and melanoma.