Post hoc gene aggregate analysis of the discovery data, including the rarest variants (minor allele count ≥ 1), identified 4 additional genes associated with DKD (P < 3.4 × 10−6; Supplementary Table S13), including EMILIN1, a kidney extracellular matrix protein that negatively regulates TGF-β signaling. The gene discussed is TGFB1; the disease is diabetic kidney disease.