In another KRYSTAL-2 study comparing pre-treatment and post-resistance samples from 38 patients, PTEN alterations were acquired in 2 patients, both of whom had colorectal cancer and exhibited additional alterations in the RTK/RAS/MAPK/PI3K pathway, suggesting the potential importance of PTEN LOF mutations in acquired resistance to adagrasib.30 Here, PIK3CA is linked to colorectal cancer.