GCG and Hyperglycemia: In sequential glucose-dependent glucagon secretion experiments with isolated human islets designed to mimic the clinical study design, GLP-2 did not affect glucagon secretion during euglycemia (5.5 mmol/L) or at subsequent hypoglycemia (2 mmol/L glucose), euglycemia, or hyperglycemia (11 mmol/L glucose) (Fig. 4A-4D).