In this study, we investigated the short-, medium-, and long-term effects of Elovl1 disruption on dry eye phenotypes and meibum lipid composition using two genetically engineered mouse models: epidermis-specific Elovl1 transgene-containing Elovl1 KO (Tg-Elovl1 KO) mice and tamoxifen (Tam)-inducible Elovl1 conditional KO mice. Here, ELOVL1 is linked to Keratoconjunctivitis sicca.