In functional assays, co-culture of sorted M-MDSCs from AT3 tumor-bearing mice with anti-CD3/anti-CD28 activated T cells resulted in a lower suppressive effect of Pgdfl/flLysMCre M-MDSCs compared to Pgdfl/fl M-MDSCs on both CD4+ and CD8+ T cells (Fig. 2G). This evidence concerns the gene CD28 and neoplasm.