FXYD5 and colorectal carcinoma: Clinical studies have shown that high expression of acidosis-related markers in tumors is linked to poor prognosis, underscoring tumor acidity as an appealing target for diagnostic and therapeutic strategies.1,15,21,22 In this study, trajectory analysis via single-cell genomic data from CRC patients revealed that tumor acidosis, dysadherin expression, and CRC progression are closely linked, collectively contributing to cancer progression (Fig. 1b).