We observed substantial levels of GSDMD-dependent cell death in mT3Sf-infected CASP4–/– cells (mediated by NLRC4) and in NLRC4–/– cells (mediated by CASP4), confirming that mT3Sf::empty infection induces pyroptosis via activation of both of these two main cell death pathways. The gene discussed is NLRC4; the disease is infection.