Based on the traditional screening of thalassemia gene, an additional 16 (17.67%) cases of clinically significant variants of rare thalassemia were identified by accurate circular consensus long‐read sequencing in this study, including 12‐point variants and 4 deletion variants: HBB: (SEA)‐HPFH, HBB: c.268_281delAGTGAGCTGCACTG, HBB: (Chinese) Gγ + (Aγδβ)0, and HBA2:c.91‐93delGAG. The gene discussed is HBA2; the disease is thalassemia.