The increase in SO2 and GSH levels in the ovaries of the negative control would be a response of the body to ovarian damage induced by PCOS, including oxidative stress (characterized by high levels of MDA) and cell death [characterized by high levels of Fe2+ (marker of ferroptosis) and caspase 3 (marker of apoptosis)] because SO2 and GSH are known to be antioxidant and antiapoptotic [26, 94]. This evidence concerns the gene CASP3 and polycystic ovary syndrome.