Furthermore, confocal microscopy analysis corroborated the autophagy-modulating effects of CMSP on ESCC cells transiently expressing green fluorescent protein (GFP)-tagged LC3B, and CMSP treatment preferentially induced an increase in GFP-LC3B puncta (Fig. 2E and Fig. S2F), suggesting that CMSP increased autophagosome of ESCC cells. The gene discussed is MAP1LC3B; the disease is esophageal squamous cell carcinoma.