However, in mice lacking both CTSB and CTSL, only trypsin activity was altered, with no significant impact on disease severity or inflammatory responses following caerulein-induced AP.195 Moreover, in CTSL-deficient mice, both trypsin activity and pancreatic apoptosis were increased, yet disease severity was paradoxically reduced.194 These findings suggest that CTSL primarily antagonizes CTSB-mediated trypsin activation but does not directly influence AP outcomes. Here, CTSB is linked to alkaline phosphatase measurement.