OXTR and Anxiety: 2023). Oxytocin also reversed VPA‐induced downregulation of shank3a, shank3b, and oxtr gene expression, suggesting its potential to modulate synaptic functions and oxytocinergic signaling in ASD‐related zebrafish phenotypes. On the other hand, embryos exposed to VPA at a concentration of 100 μM show reduced hatching rates (Lee et al. 2018). Moreover, the embryonic VPA exposure induces ASD‐related behavior phenotypes, including hyperlocomotion at 6 dpf and increased anxiety‐like behavior at 6, 70, and 120 dpf (Zimmermann et al. 2015).