This prospective, cross-sectional study aimed to evaluate the association between non-steroidal anti-inflammatory drugs (NSAIDs) and anti–tumor necrosis factor-α (anti-TNF-α) therapy and retinal and choroidal thickness in patients with ankylosing spondylitis (AS) without uveitis, and to compare these findings with healthy controls. The gene discussed is TNF; the disease is uveitis.