To assess whether IFNλ subtypes exhibit differential antiviral activity against these distinct classes of viruses, we pre-treated the intestinal epithelial cells T84 cells with increasing concentrations (0.0001–300 ng/mL) of IFNλ1, IFNλ2, or IFNλ3 for 24 hours prior to infection. This evidence concerns the gene IFNL3 and infection.