VIPR1 and infection: In addition, techniques are reported which increase the infection activity of Agrobacterium: by constitutively-active mutation in Agrobacterium virG protein, which is a response regulator of signaling pathway through detecting acetosyringone to induced vir genes’ expression (this mutation alone or in combination of expression of nahG, which encodes salicylate hydroxylase) (Jeong et al. 2024; Pazour et al. 1992; Scheeren-Groot et al. 1994); and mutation in Agrobacterium repA protein, which regulates copy number of expression plasmids (Szarzanowicz et al. 2025).