IHC (Fig. 6D) showed that GZMB-positive areas occupied 64.3% ± 7.1% of the tumor in the combination group (versus 7.2% ± 1.5% in PBS, ​​*P < 0.001), and IFN-γ expression increased 5.8-fold (*​P < 0.01). This evidence concerns the gene GZMB and neoplasm.