To examine the role of astrocytic SNAT-5 in depression, pAAV.GFAP.Cre.WPRE.hGH and pAAV2.pX552ch-sgSlc38a5 were co-injected into the mPFC of CRISPR/CAS9-EGFP mice (Fig. 3M). This evidence concerns the gene GFAP and depressive symptom measurement.