We previously reported a decrease in GS activity and a downregulation of protein expression associated with the Glu-Gln cycle, including GLT-1, SNAT-1, SNAT-2, SNAT-3, and SNAT-5, in the mPFC of mice with CIS-induced depression 14, 17, 18. This evidence concerns the gene SLC38A5 and major depressive disorder.