We also found a significant association between RSPO4 methylation and diagnosis age (p < 0.001), neoplasm histologic grade (p < 0.05), Karnofsky performance score (p < 0.001), ethnicity (p < 0.005) and asthma history (p < 0.05) in patients with brain lower grade glioma (Table S4). Here, RSPO4 is linked to neoplasm.