HSPD1 and posterior cortical atrophy: At the cellular level, comparative analysis of 5 PCa cell lines versus normal prostate epithelial cells revealed significant upregulation of both HSPD1 mRNA and HSP60 protein (P < 0.05), with 22Rv1 and C4-2 cells showing the most pronounced elevation (Fig. 1I-J, Fig. S1C).