In summary, our present study has demonstrated that DYNLL1-AS1 levels were significantly higher in irradiated ESCC cells-derived EVs, which can polarize TAMs toward an M2 phenotype and upregulate expression of PD-L1 in macrophages promoting immunosuppressive phenotype and triggered tumor immune escape mechanisms through SEC22B/FOXP1 signal pathway. The gene discussed is SEC22B; the disease is esophageal squamous cell carcinoma.