The expression of the lncRNA was further confirmed by qRT‒PCR, which revealed an increased level in the livers of HFD-fed or western diet-fed mice compared with LFD-fed mice (Fig. 1c).22,23 To explore the cell-type specificity of Wee1-AS, we analyzed single-cell transcriptomic data obtained from a WD-induced MASLD mouse model (GSE156057), which included 13 key liver cell types. Here, WEE1 is linked to metabolic dysfunction-associated steatotic liver disease.