A Jonckheere–Terpstra trend test on GSE126848 confirmed a significant monotonic increase from healthy controls to MASH patients (P = 0.049), supporting the association of LNC106435.1 with disease progression.27 Additionally, we performed immunohistochemistry to assess WEE1/p-CDK1 (Y15)/CYCLIN B1 expression in liver samples from normal individuals and patients with hepatic steatosis and MASH obtained from the clinic (Supplementary Table 2). This evidence concerns the gene CCNB1 and Hepatic steatosis.