Integrated bioinformatics analyses utilizing GO and KEGG pathway enrichment indicates that acupuncture treats depression through regulating multiple signaling cascades, including the Toll-like receptor signaling pathway, NLRP3 inflammasome, MAPK/ERK, PI3K/AKT, neurotrophin, tumor necrosis factor, and NF-κB pathways. Here, BDNF is linked to depressive disorder.