AQP4 and constipation disorder: The findings indicated that CBLs and HBLs had significant potential in preventing constipation by improving physiological parameters; increasing serum levels of Gas, SP and MTL; decreasing serum levels of SS and VIP; restoring colon AQP3, AQP4, and 5-HT4R mRNA expression; improving fecal SCFAs content; and modulating gut microbiota composition by increasing Bacteroides abundance and reducing that of Akkermansia (Figure 9).