Anti-MICB-CAR-NK cells not only increased cancer cell killing but also secreted higher levels of cytokines such as IFN-γ, TNF-α, perforin, and granzyme B. By releasing perforin and granzyme B to trigger apoptosis of tumor cells, while secreting IFN-γ and TNF-α to regulate the immune microenvironment, it forms an efficient anti-tumor mechanism with multiple levels and pathways. The gene discussed is PRF1; the disease is cancer.