PCSK9 and Hypercholesterolemia: When rAAV8-D377Y-mPCSK9 mice are fed with either a WD (D12079B, Research diets Inc. containing 21% fat and 0.21% cholesterol without added sodium cholate) or Paigen diet (D12336, Research diets Inc., New Brunswick, NJ, USA, containing 16% fat, 1.25% cholesterol, and 0.5% sodium cholate), persistent hypercholesterolemia develops in a PCSK9 dose-dependent manner [64].