Given that bile acid dysregulation is closely associated with dyslipidemia and insulin resistance in MAFLD, and that we discovered that AHR deficiency may suppress bile acid synthesis, we performed comparative analysis of hepatic and fecal bile acid profiles between the WT-HFHFrHCD and AHR−/−-HFHFrHCD groups to investigate the effect of AHR knockout on hepatic and fecal bile acids. This evidence concerns the gene AHR and metabolic syndrome.