The exploratory analysis of RNA-seq by TIMER2.0 showed a positive correlation between CHRM3 and PD-L1 in both LGG (p < 0.0001) and GBM (p = 0.02), whereas no correlation was observed between CHRM1, ChAT, or AChE, showing in all cases a wider dispersion of the analyzed data (Figure 5H,I). The gene discussed is ACHE; the disease is glioblastoma.