Therefore, we injected the virus AAV-CaMKII-hM4Di-mCherry carrying inhibitory chemogenetic components and the control virus AAV-CaMKII-mCherry into the DG of mice, and examined the viral expression (Figure 4C), to investigate whether inhibiting the activity of excitatory neurons in the DG alters the mild cognitive impairment phenotype in mice subjected to the ASCV model. Here, CAMK2G is linked to Cognitive impairment.