PFKFB3 and malignant colon neoplasm: Within the CRC cohort, the CC subgroup maintained significantly higher PFKFB3 expression than RC (1.4-fold increase; 34.50 ± 27.37 vs. 24.72 ± 21.53, p < 0.045), corroborating prior monocyte data and underscoring a specific role for PFKFB3 in colon cancer pathogenesis [13].