APOE and age-related macular degeneration: These 3D cultures can be maintained up to 360 days and develop several AMD-like features, including increased autofluorescence, lipid droplet accumulation, calcification, and extracellular aggregates enriched in drusen-associated proteins, such as apolipoprotein E (APOE) and tissue inhibitor of metalloproteinases-3 (TIMP3).