Tgfb1 (Transforming growth factor beta 1) gene, that regulates TGF-β, a master regulator of fibrogenesis and central to systemic sclerosis (SSc) pathogenesis, was significantly upregulated at day 45 post-immunization (IMU-COLV45) compared to both IMU-COLV15 and IMU-COLV30 (2.46 ± 0.81 vs. 0.93 ± 0.64, p < 0.0001; vs. 1.16 ± 0.84, p = 0.0003) (Figure 5A). This evidence concerns the gene TGFB1 and systemic sclerosis.