To determine optimal conditions for subsequent therapeutic studies, B16 ffLuc GFP EGFR tumor cells were implanted intradermally (i.d.)into female C57BL/6 mice at doses of 0.5 × 106, 1 × 106, or 2 × 106 cells per mouse (n = 3 per group). This evidence concerns the gene EGFR and neoplasm.