EGFR and neoplasm: Co-culture with B16 ffLuc GFP EGFR cells for 24 h induced the secretion of approximately 4 pg/mL of IL-2 and 3.5 × 103 pg/mL of interferon-gamma (IFN-γ) by EGFR mCAR T cells, while no significant cytokine release was detected in cultures without antigen-positive tumor cells (Figure 3F).