ANOVA on GAD67 protein levels showed a significant effect of CRS duration (ANOVA: F(5,81) = 2.9; p = 0.017), and no effect of sex or CRS duration*sex interaction (Figure 2H), explained by reduced expression levels in the CRS14, CRS21, and CRS28 groups (p < 0.05). Here, GAD1 is linked to congenital rubella syndrome.