Se-Chan Kim et al. treated healthy rat cardiomyocytes with HSP60 purified from injured rat cardiomyocytes and confirmed that extracellular HSP60 mediates apoptosis via toll-like receptor (TLR)-4, suggesting that HSP60-mediated activation of TLR4 may be a mechanism for cardiomyocytes loss in HF [106]. This evidence concerns the gene TLR4 and hydrops fetalis.