Through GO analysis, KEGG pathway analysis, and protein–protein interaction network screening, key signaling pathways related to HOXB9 and TMZ-A2SLN were identified, such as the PPAR signaling pathway associated with tumor drug resistance, the NF-κB signaling pathway linked to inflammation, and the Hippo signaling pathway involved in cancer suppression. Here, HOXB9 is linked to neoplasm.