We used the Annexin V-FITC/PI apoptosis detection method to investigate apoptosis of glioma cells under different conditions (groups: PBS, Free TMZ, siHOXB9, TMZ-A2SLN, TMZ-A2SLN + NCsiRNA, TMZ-A2SLN + siHOXB9). The gene discussed is ANXA5; the disease is glioma.