In this study, we aimed to determine the effects of HSP-27, Caspase-3, IL-2, γ-H2AX, HMGB-1, SP-D, and GDH on the pathogenesis of rotavirus infection by using biomarkers of lung and liver injury in neonatal diarrheic calves naturally infected with rotavirus, both in vivo and postmortem. This evidence concerns the gene HMGB1 and Rotavirus infection.