Figure 7A demonstrates that direct contact between THP1 and MDA-MB-231 cells for 24 h resulted in a significant increase in secreted IL6 protein levels compared to individual monocultures (~935 fold vs. THP1 and 2.7-fold vs. MDA-MB-231 alone). Figure 7B demonstrates a striking induction (~3000-fold increase) of intracellular IL6 protein levels in cell lysates in co-cultured THP1 cells, further confirming activation of IL6 expression in response to tumor–immune cell communication. This evidence concerns the gene IL6 and neoplasm.