To further investigate whether the EGFR and pAKTSer473 interaction observed in vitro also occurs in vivo, A549-Puro and A549-BRG1 tumor tissues treated with DMSO or gefitinib were stained for EGFR and pAKTSer473 complex formation, and positivity in different cellular compartments (membrane, cytoplasmic, and nuclear) of stained tissues was assessed and quantified. Here, EGFR is linked to neoplasm.