Additionally, single-sample gene set enrichment analysis (ssGSEA) was utilized to explore the association between AGPAT4 expression and the level of immune infiltration in the tumor microenvironment.<h4>Results</h4>Our findings revealed that AGPAT4 was significantly downregulated in thyroid cancer (THCA) tissues (P < 0.001), suggesting a potential tumor-suppressive role of AGPAT4 in thyroid cancer. This evidence concerns the gene AGPAT4 and thyroid cancer.