In addition, we performed IHC analysis to evaluate the expression levels of NF-κB (p65) in xenograft subcutaneous tumor, and the results showed that the expression of NF-κB (p65) was significantly decreased in the sh-RECQL4 group, while the opposite result was observed in the oe-RECQL4 group (Supplementary Fig. 5). This evidence concerns the gene NFKB1 and neoplasm.