The lack of Tmem55b led to hepatic steatosis in vivo (Fig. 1A–C), an effect we confirmed after TMEM55B knock-down in Huh7 cells (Supplementary Fig. S8B), incubated with 1 mM oleic acid and stained cells with Nile Red, a neutral lipid dye. This evidence concerns the gene PIP4P1 and fatty liver disease.