Notably, in multivariable logistic regression, GPC-3 was independently associated with HCC (OR = 1.01, 95% CI 1.00–1.01; p < 0.001), whereas AFP was not (OR = 1.00, 95% CI 0.99–1.00; p = 0.115). Here, GPC3 is linked to hepatocellular carcinoma.