MRC1 and colorectal carcinoma: The results revealed a significantly higher proportion of F4/80 and CD206 double-positive M2-like macrophages in the TIMP1-overexpression group compared with controls (p < 0.05), suggesting that CRC-derived TIMP1 promotes the accumulation of M2-like macrophages within the liver immune microenvironment to establish a pre-metastatic niche (Figure 5E).