Furthermore, activation of spinal TLR7 by incubating normal spinal cords (n = 4) with DSR 6434 (2 μM) for 2.5 h significantly increased Cav2.2 expression in the dorsal horn (Figure 5B), reproducing the pathological change observed in lupus mice. This evidence concerns the gene TLR7 and systemic lupus erythematosus.